Integrative Single‐Cell Analysis Reveals Iron Overload‐Induced Senescence and Metabolic Reprogramming in Ovarian Endometriosis‐Associated Infertility

作者：Yangshuo Li, Wei Zhou, Jie Ding, Di Song, Wen Cheng, Jin Yu, Shuai Sun, Shanshan Mei, X Liang, Qianqian Zhao, Yanping Kuang, Ming‐Qing Li, Zhexin Ni, Chaoqin Yu, Yue Gao · 发表于：Advanced Science · 年份：2025 · DOI：10.1002/advs.202417528 · 被引用次数：5 · 研究领域：Endometriosis Research and Treatment、Reproductive System and Pregnancy、Reproductive Biology and Fertility

Endometriosis, particularly ovarian endometriosis (OE), is a major cause of infertility, often associated with reduced oocyte quality and impaired ovarian function. Iron overload plays a key role in OE progression. This study investigates the effects of iron overload on follicular function in OE-associated infertility (OEI). A single-cell atlas of pre-ovulatory follicular fluid from OEI patients reveals dynamic changes in iron metabolism and iron-induced senescence phenotypes. Spatial transcriptomics using Stereo-seq in iron-overloaded mouse ovaries further identifies localized senescence features. Additional analysis of aging human ovaries highlights conserved patterns of iron dysregulation. These findings provide mechanistic insight into iron overload-related ovarian pathology and suggest potential therapeutic targets for improving oocyte quality in OEI.