Study on the targets and mechanisms of 7-hydroxyethyl chrysin in prevention and treatment of high-altitude cerebral edema using proteomics technology

作者：Dongmei ZHANG, Xiaolin Li, Chenyu YANG, Linlin Jing, Lei He, Huiping Ma · 发表于：Journal of Zhejiang University (Medical Sciences) · 年份：2025 · DOI：10.3724/zdxbyxb-2024-0291 · 研究领域：High Altitude and Hypoxia、Cancer, Hypoxia, and Metabolism、Flavonoids in Medical Research

Objective To investigate the targets and mechanism of 7-hydroxyethyl chrysin (7-HEC) in prevention and treatment of high-altitude cerebral edema (HACE) in rats. Methods Fifty-four male Wistar rats were randomly divided into normal control group, HACE model group, and 7-HEC-treated group (18 rats in each group). Except for the normal control group, rats in the other two groups were exposed to a hypobaric hypoxic chamber simulating 7000 m altitude for 72 h to establish the HACE model. The 7-HEC-treated group received intraperitoneal injection of 7-HEC (150 mg·kg－¹·d－¹) for 3 days prior to modeling, while the model group received equivalent saline. Tandem Mass Tag (TMT) proteomics technology was used to detect differentially expressed proteins (DEPs) with screening criteria set at a fold change >1.2 and P<0.05. Western blotting was used to verify the expression levels of target proteins. Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and protein-protein interaction (PPI) network analysis were performed. Results Compared with the normal control group, 256 DEPs were identified in the HACE model group. Com-pared with the HACE model group, 87 DEPs were identified in the 7-HEC-treated group. Among them, 19 DEPs that were dysregulated in the HACE model group were restored after 7-HEC intervention, of which seven (HSPA4, Arhgap20, SERT, HACL1, CCDC43, POLR3A, and PCBD1) were confirmed by Western blotting. GO enrichmen...