Novilysobacter viscosus sp. nov. and Novilysobacter longmucuonensis sp. nov., two novel species capable of producing proteases and RiPPs

作者：Dorji Phurbu, Xuan Zhang, Rui Wang, Zi-Xuan Liu, Yanyan Zheng, Ai-Hua Li · 发表于：BMC Microbiology · 年份：2025 · DOI：10.1186/s12866-025-04178-y · 被引用次数：2 · 研究领域：Enzyme Production and Characterization、Genomics and Phylogenetic Studies、Microbial Metabolism and Applications

As prolific producers of extracellular lytic enzymes and antimicrobial natural products, Lysobacter strains have garnered great interest over the past decade. Three gram-stain-negative, aerobic, short rod bacterial strains with flagella, designated A3-1-A15T, D1-1-M9T and D1-1-M8, were isolated from the shore soil of Longmucuo Lake in the Xizang Autonomous Region, China. Comparisons of the 16 S rRNA gene sequences confirmed their affiliation with the genus Novilysobacter. Strain A3-1-A15T presented the highest similarity of the 16 S rRNA gene sequence to Novilysobacter selenitireducen 13AT (97.75%), whereas strains D1-1-M9T and D1-1-M8 were most closely related to Novilysobacter erysipheiresistens RS-LYSO-3T, showing 97.80% similarity. The genomic DNA G + C contents of strains A3-1-A15T, D1-1-M9T and D1-1-M8 were 67.46%, 68.79% and 67.85%, respectively, based on the genome sequence data. The ANI values of strains A3-1-A15T and D1-1-M9T with N. selenitireducens A13T and N. erysipheiresistens CICC 23,922T were 84.45%/78.03% and 79.41%/79.81%, respectively. Corresponding dDDH values were 49.7%/23.2% and 24.7%/25.4%, all below the species thresholds, confirming two novel Novilysobacter species. The main polar lipids included diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The major cellular fatty acids were summed feature 9 (C16:0 10-methyl and/or iso-C17:1ω9c), iso-C15:0, iso-C16:0, iso-C11:0 and iso-C11:0 3-OH. Protease production was confirmed throug...