Integrin CD103 reveals a distinct developmental pathway of autoreactive thymocytes in TCR transgenic mice

作者：Nurçin Liman, Can Li, Megan A. Luckey, Hilary R. Keller, Jie Li, William Hajjar, Jan Wiśniewski, Michael J. Kruhlak, Vanja Lazarevic, Jung‐Hyun Park · 发表于：Nature Communications · 年份：2025 · DOI：10.1038/s41467-025-61922-8 · 被引用次数：3 · 研究领域：T-cell and B-cell Immunology、Immunotherapy and Immune Responses、Immune Cell Function and Interaction

Clonal deletion through negative selection is critical to eliminate autoreactive T cells in the thymus. Negative selection, however, is imperfect such that some autoreactive thymocytes can escape thymic deletion and successfully populate peripheral tissues. This is also the case for autoreactive 2D2 TCR transgenic T cells, a widely employed mouse model in studying the pathogenesis of CD4 T cell-mediated experimental autoimmune encephalomyelitis. How autoreactive 2D2 thymocytes evade negative selection, however, remains incompletely understood. Here we show that negative selection of MHC-II-restricted thymocytes, specifically 2D2 TCR transgenic T cells, is associated with the induction of integrin CD103, and that forced expression of CD103 downregulates CXCR4 expression, alters intra-thymic trafficking, and reinforces clonal deletion of immature thymocytes. Stratification of positively versus negatively selected 2D2 T cells based on their distinct coreceptor expression further shows that CD103 does not affect the generation of conventional CD4 T cells but is deleterious for autoreactive CD4, CD8 double-negative 2D2 T cells that correspond to CD69-negative CCR7-intermediate thymocytes, displaying markers of agonistic TCR signalling. Collectively, these results propose CD103 expression as an indicator and contributor of negative selection for MHC-II-restricted T cells, providing further mechanistic insights into the process of T cell selection in the thymus.