Probiotic efficacy of Cetobacterium somerae (CGMCC No. 28843): promoting intestinal digestion, absorption, and structural integrity in juvenile grass carp (Ctenopharyngodon idella)

作者：Yuanxin Chen, Wei‐Dan Jiang, Pei Wu, Yang Liu, Yao-Bin Ma, Hongmei Ren, Xiao‐Wan Jin, Jun Jiang, Ruinan Zhang, Hua Li, Lin Feng, Xiao‐Qiu Zhou · 发表于：Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · 年份：2025 · DOI：10.1186/s40104-025-01224-7 · 被引用次数：15 · 研究领域：Aquaculture disease management and microbiota、Aquaculture Nutrition and Growth、Gut microbiota and health

BACKGROUND: Cetobacterium somerae, a symbiotic microorganism resident in various fish intestines, is recognized for its beneficial effects on fish gut health. However, the mechanisms underlying the effects of C. somerae on gut health remain unclear. In this experiment, we investigated the influence of C. somerae (CGMCC No.28843) on the growth performance, intestinal digestive and absorptive capacity, and intestinal structural integrity of juvenile grass carp (Ctenopharyngodon idella) and explored its potential mechanisms. METHODS: A cohort of 2,160 juvenile grass carp with an initial mean body weight of 11.30 ± 0.01 g were randomly allocated into 6 treatment groups, each comprising 6 replicates (60 fish per replicate). The experimental diets were supplemented with C. somerae at graded levels of 0.00 (control), 0.68 × 10⁹, 1.35 × 10⁹, 2.04 × 10⁹, 2.70 × 10⁹, and 3.40 × 10⁹ cells/kg feed. Following a 10-week experimental period, biological samples were collected for subsequent analyses. RESULTS: Dietary supplementation with C. somerae at 1.35 × 10⁹ cells/kg significantly enhanced growth performance, intestinal development, and nutrient retention rate in juvenile grass carp (P < 0.05). The treatment resulted in increased intestinal acetic acid concentration and enhanced activities of digestive enzymes and brush border enzymes (P < 0.05). Furthermore, it reduced intestinal permeability (P < 0.05), preserved tight junctions (TJ) ultrastructural integrity, and increased the express...