Shenling Tongqiao powder targets TGF-β1/Smad-Wnt/β-catenin axis to suppress epithelial-mesenchymal transition in chronic rhinosinusitis

作者：Kai Liu, Hui Zeng, Xiao Zhu, Hao Deng, Wei Deng, Sixin Li, Yaxian Zhu, Bin Ji, Shuai Lü, Wei Wu, Qi Wu, Jun Liao, Hu Ge, Wei Chang, Y S Ying, Ying Wang, Zhenhua Zhu · 发表于：Phytomedicine · 年份：2025 · DOI：10.1016/j.phymed.2025.157065 · 被引用次数：5 · 研究领域：Sinusitis and nasal conditions、Allergic Rhinitis and Sensitization、Nasal Surgery and Airway Studies

BACKGROUND: Chronic rhinosinusitis (CRS), a prevalent upper respiratory inflammatory disorder, remains challenging in clinical management due to the high recurrence rates. Traditional Chinese Medicine (TCM) pathogenesis theory posits that CRS primarily stems from deficiency syndromes involving pulmonary and splenic dysfunction. Shenling Tongqiao Powder (SLTQP), a formulated herbal preparation, has demonstrated clinical efficacy in enhancing pulmonary-splenic function and CRS symptom alleviation. But its underlying pharmacological mechanisms require systematic elucidation. METHODS: Nasal mucosa of nasal septum deviation and CRS without nasal polyp (CRSsNP) patients were collected for histopathological analysis in epithelial-mesenchymal transition (EMT). The rat model of CRS was established using Staphylococcus aureus while the body weight, the number of nose-scratching and sneezing were recorded. Sinus inflammation was evaluated by computed tomography (CT) scanning. Histopathological staining, Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR) and western blot were applied for assessment of TGF-β1/Smad and Wnt/β-catenin signaling and EMT in rat and cell model. Inflammatory factors of rat nasal lavage solution and serum were analyzed by ELISA. LC-MS/MS was used for analysis of SLTQP decoction and pharmaceutic serum. The energy between these molecules and TGF-β1/Smad and Wnt/β-catenin was calculated by molecular docking, the direct binding affinity ...