Kaempferol drives genotype-specific microbiota Bacillaceae to enhance nitrogen acquisition in rapeseed

作者：Youqiang Wang, Dong‐Lin Zhao, Zhe Li, Yanzhe Yang, Zhi-Xin Peng, Chen Meng, Siqi Ma, Kangwen Xu, Yiqiang Li, Guangda Ding, Yanfen Zheng, Chengsheng Zhang · 发表于：Journal of Advanced Research · 年份：2025 · DOI：10.1016/j.jare.2025.07.018 · 被引用次数：12 · 研究领域：Plant-Microbe Interactions and Immunity、Plant nutrient uptake and metabolism、Legume Nitrogen Fixing Symbiosis

INTRODUCTION: Host genotype is a key driver in shaping plant microbiome in response to dynamic changes in soil nitrogen (N) availability. However, the effects of rapeseed (Brassica napus) genotypes with different N use efficiency characteristics on microbiome assembly, as well as the underlying plant-microbe interaction mechanisms, remain poorly understood. OBJECTIVES: This study aims to: (1) assess microbial assembly differences between N-use efficient and inefficient genotypes; (2) identify specific microbiota associated with plant N acquisition; and (3) reveal the molecular mechanisms driving plant-microbe interactions. METHODS: We conducted comparative microbiome profiling of N-use efficient and inefficient genotypes, followed by functional validation of microbial roles in plant N uptake. Multi-omics approaches, including RNA-seq and metabolomics, were used to uncover the regulatory interactions between the host and rhizosphere microbiota. RESULTS: N tracer assays. RNA-Seq analysis further demonstrated that genes involved in jasmonic acid and ethylene signaling pathways were upregulated in strain 41S2-inoculated plants, likely contributing to enhanced root development. Metabolomic profiling identified kaempferol, a flavonol with the highest fold-change between genotypes, as a key root exudate promoting the growth and biofilm formation of strain 41S2. Furthermore, the fls1 mutant (deficient in kaempferol biosynthesis) failed to recruit Bacillaceae, confirming the role of k...