The Primary Cultivation of Oogonial Stem Cells from Black Rockfish (Sebastes schlegelii): Morphology and Transcriptome Landscape

作者：Jingjing Zhang, Lei Lin, Shengyu Zhu, Yanming Zhang, Caichao Dong, Yang Yu, Yuyan Liu, Xuwen Cao, Yangbin He, Hong‐Long Ji, Bo Meng, Qian Wang, Changwei Shao · 发表于：International Journal of Molecular Sciences · 年份：2025 · DOI：10.3390/ijms26146772 · 被引用次数：5 · 研究领域：Animal Genetics and Reproduction、MicroRNA in disease regulation、Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities

Black rockfish (Sebastes schlegelii) is a marine ovoviviparous teleost that exhibits significant sexual dimorphism, with females growing faster and reaching larger sizes than males. Establishing stable oogonial stem cells (OSCs) is critical for understanding germline stem cell dynamics and facilitating all-female breeding. In this study, we successfully isolated and cultured OSCs from S. schlegelii for 12 passages. These cells exhibited alkaline phosphatase activity, expressed germline marker genes (ddx4, cdh1, klf4), and maintained a diploid karyotype (2n = 48). Transcriptomic comparisons between early (P3) and late (P12) passages revealed significant metabolic dysfunction and cell cycle arrest in the late-passage cells. Specifically, the down-regulation of glutathione-related and glycolysis-related genes (gstm3, gstt1, mgst3, gsta1, gsta4, gsto1, gapdh) and key mitotic regulators (cdk1, chk1, cdk4, e2f3, ccne2, ccnb1) suggested that metabolic imbalance contributes to oxidative stress, resulting in cell cycle inhibition and eventual senescence. This study provides a marine fish model for investigating metabolism-cell cycle interactions in germline stem cells and lays the foundation for future applications in germ cell transplantation and all-female breeding.