Self-Assembled Nanoplatform with pH/NIR Light-Responsive Drug Delivery for Combined Therapy of Glioma in vitro

作者：Zhiping Cao, Haiyan Gao, Yong Xu · 发表于：International Journal of Nanomedicine · 年份：2025 · DOI：10.2147/ijn.s531102 · 被引用次数：6 · 研究领域：Nanoplatforms for cancer theranostics、Nanoparticle-Based Drug Delivery、Cancer Research and Treatments

Purpose: The integration of multifunctional components into a single nanoplatform offers significant potential for personalized and minimally invasive therapeutic applications. Herein, we present a facile yet versatile strategy to engineer a near-infrared (NIR) pH- and light-responsive nanomaterial, demonstrating promising efficacy in glioblastoma combination therapy through multimodal synergistic mechanisms. Methods: The FEID nanoplatform was engineered by co-assembling ICG, DOX, and Fe³⁺. Systematic characterization included physicochemical properties (TEM, DLS, UV-vis-NIR spectroscopy, zeta potential measurements, and XPS), photothermal conversion efficiency, Fenton reaction kinetics, PTT-enhanced CDT performance, laser-triggered drug release patterns, and NIR-responsive drug release and dual-modal imaging (fluorescence/MRI) capabilities. Intracellular DOX accumulation and ROS generation were confirmed by confocal laser scanning microscopy and flow cytometry in U87 glioma cells. Hemolysis assay, cytotoxicity profiling against normal 293T and RAW264.7 cells, and H&E staining were applied for biosafety assessment. The synergistic anti-glioblastoma efficacy was systematically evaluated through MTT assays, live/dead cell staining, and apoptosis detection via Annexin V/PI staining. The pharmacokinetic profiles and blood-brain barrier (BBB) permeability of the FEID were analyzed in vivo. Results: The FEID nanoplatform displayed uniform spherical morphology (78.2 nm average diame...