Mn-doped cerium dioxide nanozyme mediates ROS homeostasis and hormone metabolic network to promote wheat germination under low-temperature conditions

作者：Yixin Wu, Xu Shen, Mengqing Sun, Hui Wei, Muhammad Faheem, Lizhu Liu, Gaoling Shi, Yan Gao · 发表于：Chemical and Biological Technologies in Agriculture · 年份：2025 · DOI：10.1186/s40538-025-00816-9 · 被引用次数：9 · 研究领域：Advanced Nanomaterials in Catalysis、Carbon and Quantum Dots Applications、Nanoparticles: synthesis and applications

Nanozymes are a class of nanocatalytic materials that mimic the functions of natural enzymes. Their enzyme-like properties enable the catalytic scavenging of excess reactive oxygen species (ROS) generated in plants under abiotic stress, thereby alleviating oxidative stress. Research on nanozymes’ role and related mechanisms in alleviating low-temperature stress in crops is still unclear. Therefore, developing nanozymes that enhance early stage cold tolerance in crops is critical for maintaining agricultural production and global food security. We synthesized a nanozyme with catalase (CAT)-like activity, manganese-doped cerium oxide (MCNPs) nanoparticles. This study demonstrates that priming with MCNPs significantly accelerated wheat germination under cold stress, increasing the germination index by 7.1% and seedling biomass by 6.2–17.2% compared to hydropriming. Through SP–ICP–MS analysis, we confirmed that MCNPs can enter the seed. We also found that the catalase-like activity of MCNPs synergistically enhanced endogenous antioxidant enzymes (CAT and superoxide dismutase) to effectively eliminate excessive ROS in wheat seeds. Further analysis using LC–MS and qPCR showed that this ROS homeostasis influenced hormone metabolism by regulating the expression of genes involved in the hormone metabolic network, elevating growth-promoting hormones (gibberellin and ethylene) by 25.5–27.2% while suppressing stress-responsive hormones (jasmonic acid and abscisic acid). Subsequent activa...