Organic Materials Promote Soil Phosphorus Cycling: Metagenomic Analysis

作者：Wei Yang, Yue Jiang, Jiaqi Zhang, Wei Wang, Xuesheng Liu, Yu Jin, Sha Li, Juanjuan Qu, Yuanchen Zhu · 发表于：Agronomy · 年份：2025 · DOI：10.3390/agronomy15071693 · 被引用次数：7 · 研究领域：Phosphorus and nutrient management、Soil Carbon and Nitrogen Dynamics、Wastewater Treatment and Nitrogen Removal

The combined application of chemical fertilizers with organic materials contributes to higher contents of bioavailable phosphorus. However, the underlying mechanism remains poorly understood. A field experiment including four treatments, chemical fertilizer (CF), chemical fertilizer with biochar (CB), chemical fertilizer with organic fertilizer (CO), and chemical fertilizer with biochar and organic fertilizer (CBO), was conducted to explore how the combination of fertilizer applications enhanced soil phosphorus bioavailability using metagenomic sequencing technology. The results showed that chemical fertilizers combined with organic materials (CB, CO, and CBO) significantly increased citrate-extractable phosphorus by 34.61–138.92% and hydrochloric acid-extractable phosphorus contents by 72.85–131.07% compared to CF. In addition, the combined applications altered the microbial community structure and increased the abundance of phoR, spoT, and ppnK genes, but decreased those of gcd, phoD, and ppk1 genes. A partial least squares path model indicated that the combined applications regulated the microbial community composition and gene abundance of phosphorus-cycling microorganisms by influencing soil physicochemical properties, thereby enhancing soil phosphorus cycling. Correlation analysis indicated that pH, total phosphorus, and available phosphorus were the key factors influencing microbial communities, while available nitrogen and total nitrogen primarily regulated phosphorus...