A virulent phage vB_VpaP_R28Z infecting Vibrio parahaemolyticus with potential for therapeutic application

作者：Huiying Zhong, Wenjie Huang, Kate Ting Lin, Qian Zhang, Yuchan Deng, Rui Zhang, Ruijie Ma · 发表于：BMC Microbiology · 年份：2025 · DOI：10.1186/s12866-025-04133-x · 被引用次数：4 · 研究领域：Bacteriophages and microbial interactions、Invertebrate Immune Response Mechanisms、Aquaculture disease management and microbiota

Vibrio parahaemolyticus , a major foodborne pathogen, contaminates aquatic products and causes significant economic losses in aquaculture while threatening food security and public health. Given the rising prevalence of multidrug-resistant V. parahaemolyticus strains, phage therapy has emerged as an effective and environmentally sustainable alternative for controlling vibriosis in aquaculture. In this study, a virulent bacteriophage, vB_VpaP_R28Z (R28Z), which infects V. parahaemolyticus ATCC 17802 T , was isolated and comprehensively characterized. R28Z was isolated from sewage collected at a seafood market in southern China and exhibits pronounced lytic properties against pathogenic Vibrio species prevalent in aquaculture environments, including Vibrio parahaemolyticus , Vibrio diabolicus , and Vibrio alginolyticus . Transmission electron microscopy (TEM) analysis revealed that R28Z belongs to the podophage morphotype. Genomic analysis indicated that R28Z is a double-stranded DNA phage with a genome size of 43,284 bp and a G + C content of 49.34%. Notably, no genes associated with lysogeny, virulence, or antibiotic resistance were detected in R28Z, suggesting its suitability for therapeutic applications. R28Z demonstrated high efficacy in lysing host cells, with a short latent period of less than 10 min and a large burst size of 113 ± 19 PFU/cell (average ± s.d.). Additionally, R28Z exhibited stability over a broad pH range of 4–10 and at temperatures ranging from 4 °C to 5...