Study on Dehydroabietic Acid-Based Schiff Derivatives from Natural Rosin as Potential Antifungal Agents

作者：Renle Xu, Jianglei Ma, Runhe Huang, Feng Gu, Yanqing Gao, Zhanqian Song, Shibin Shang, Jian Li · 发表于：Journal of Agricultural and Food Chemistry · 年份：2025 · DOI：10.1021/acs.jafc.5c01219 · 被引用次数：6 · 研究领域：Biological Activity of Diterpenoids and Biflavonoids、Phytochemicals and Antioxidant Activities、Free Radicals and Antioxidants

To develop an environmentally friendly rosin-based fungicide for controlling fusarium crown rot, a series of dehydroabietyl-Schiff-based derivatives were designed and synthesized. All compounds were validated by FT-IR, 1 H NMR, 13 C NMR, and LC–MS spectroscopies. In vitro activity assays showed that compound 4o (Co. 4o) significantly inhibited the growth of Fusarium pseudograminearum (half-maximal effective concentration, EC 50 = 5.45 μg/mL), and in vivo activity measurement results suggested that Co. 4o exhibited a certain protective effect on wheat plants. Mycelial morphological observations revealed that Co. 4o produced severe damage to the mycelial surface morphology and ultrastructure. Reactive oxygen species (ROS) staining indicated that treatment with Co. 4o led to the accumulation of substantial ROS in the mycelium. Physiological assays indicated Co. 4o significantly inhibited superoxide dismutase, peroxidase, catalase, and polyphenol oxidase protein activity. Molecular docking studies suggested that the Schiff-based group in Co. 4o could form hydrogen bonds with various amino acids. In summary, these results demonstrate that Co. 4o holds potential as an antifungal candidate for controlling fusarium crown rot.