Replicability of Scientific Claims in Drosophila Immunity: A Retrospective Analysis of 400 Publications

作者：Hannah E. Westlake, Fabrice David, Yao Tian, Kenan Krakovic, Prince Kumar Sah, Nathan Klotz, Asya Dolgikh, Liza Juravlev, Thomas Esmangart de Bournonville, Alexia Carboni, Claudia Melcarne, Tisheng Shan, Yang Wang, Yizhu Mu, Akshata Kotwal, Nadia Pirko, Jean Philippe Boquete, Fanny Schüpfer, Samuel Rommelaere, Mickaël Poidevin, Zhonggeng Liu, Shu Kondo, Girish S. Ratnaparkhi, Sveta Chakrabarti, Xiaoxue Li, Florent Masson, Xiaoxue Li, Mark A. Hanson, Haobo Jiang, Francesca Di Cara, Estee Kurant, Bruno Lemaître · 发表于：bioRxiv (Cold Spring Harbor Laboratory) · 年份：2025 · DOI：10.1101/2025.07.07.663442 · 被引用次数：8 · 研究领域：Invertebrate Immune Response Mechanisms、Insect symbiosis and bacterial influences、Neurobiology and Insect Physiology Research

Abstract Drosophila immunity has been the focus of intense study and has impacted other research fields including innate immunity and agriculturally or epidemiologically relevant investigations of insect pests and vectors. Unsurprisingly for such a large body of work, some published results were later found to be irreplicable. Although some results have been contradicted in the literature, many have no published follow-up, either due to a lack of research or low motivation to publish negative or contradictory results. We have addressed this by performing a reproducibility project that analyses the conceptual replicability of claims from articles published on Drosophila immunity before 2011. To assess replicability, we extracted claims from 400 articles on the Drosophila immune response to bacteria and fungi and performed preliminary verification by comparing these claims to other published literature in the field. Using alternative approaches, we also experimentally tested some ‘unchallenged’ claims, with no published follow-up. The intent of this analysis was to centralize evidence on insights and findings to improve clarity for scientists that may base research programs on these data. Although the aim of the ReproSci project is to assess the replicability of claims made in articles published in the field of Drosophila immunity, it is in no way an assessment of the ‘scientific value’ of the research. All our data are published on a publicly available website associated with ...