Identifying propionate metabolism-related genes as biomarkers of sepsis development and therapeutic targets

作者：Lechen Yang, Weifeng Shang, Dongjie Chen, Dongjie Chen, Hang Qian, Sheng Zhang, Xiaojun Pan, Sisi Huang, Jiao Liu, Dechang Chen, Dechang Chen · 发表于：Scientific Reports · 年份：2025 · DOI：10.1038/s41598-025-06463-2 · 被引用次数：4 · 研究领域：Cancer, Hypoxia, and Metabolism、Diet and metabolism studies、Hyperglycemia and glycemic control in critically ill and hospitalized patients

The treatment of sepsis is challenging due to unclear mechanisms. Propionate is increasingly seen as critical to sepsis pathophysiology by bridging gut microbiota and immunity, but the mechanisms remain unclear. Our study analysed differences in propionate metabolism in peripheral blood mononuclear cells from septic patients and healthy controls using single-cell RNA-seq (scRNA-seq) data. Differentially expressed genes (DEGs) analysis, pathway enrichment, transcription factor (TF) prediction, intercellular communication, and trajectory inference were used to explore the role of propionate metabolism in sepsis. We constructed a sepsis diagnostic model using LASSO and machine learning (XGBoost, CatBoost, NGBoost) with bulk RNA-seq data. scRNA-seq analysis revealed that propionate metabolism was highest in plasma cells (PCs), which can be classified into high and low metabolism groups, identifying 9,155 DEGs. High propionate metabolism was associated with metabolism such as short-chain fatty acids, while low metabolism was related to negative regulation of wound healing. The DoRothEA regulator algorithm showed TFs such as IRF4, ARID3A, FOXO4, and ATF2 were activated in high propionate metabolism subgroups, whereas NR5A1, BCL6, and CDX2 were activated in low subgroups. Cell-cell communication revealed that both groups interacted primarily with B cells and neutrophils, with the high propionate metabolism PCs showing more significant interactions. The receptor-ligand pairs primaril...