A Novel Hand-Held Spinning Platform with Centrifugal Microfluidics for Rapid, Cost-Effective Urinary Total Protein Detection at the Point of Care

作者：Wei Li, Jing Zhang, Junchen Liao, Min Zhu, Xinrui Wang, Xiang Zhou, Zhiqiang Ma, Mohamed Elhousseini Hilal, Song Lin Chua, Guangming Tan, Bryan P. Yan, Bee Luan Khoo · 发表于：Analytical Chemistry · 年份：2025 · DOI：10.1021/acs.analchem.5c00930 · 被引用次数：5 · 研究领域：Biosensors and Analytical Detection、Advanced Proteomics Techniques and Applications、Single-cell and spatial transcriptomics

High Resolution Image Download MS PowerPoint Slide Urinary total protein (UTP) serves as a critical biomarker for diagnosing and managing various diseases; however, current detection methods often face limitations regarding accessibility, cost, and operational complexity for point-of-care applications. This study presents a novel hand-held spinning platform with centrifugal microfluidics (HSP-C M ), a groundbreaking technology designed for electricity-free, cost-effective UTP detection at a remarkably low cost of approximately 1.4 USD per test. The HSP-C M integrates a 3D-printed spinning device that ensures uniform reagent distribution and a centrifugal microfluidic biochip capable of providing quantitative UTP analysis in a colorimetric, rapid (∼3 min), and user-friendly format. Notable innovations of the system include tunable hydrophilic–hydrophobic surface properties via microchannel aspect ratio adjustments, optimized optical conditions with an ultrafast exposure time of 1/100 s, and minimal sample volume requirements (5 μL), resulting in a detection limit as low as 3.49 μg/mL. The system demonstrated high throughput (n = 16) and strong correlation (0.9481) and agreement (90.32%) with gold-standard laboratory methods when validated using clinical urine samples from both healthy donors (n = 6) and myocardial infarction (MI) patients (n = 25). Furthermore, the HSP-C M successfully identified clinically relevant UTP thresholds (>63.11 μg/mL) for distinguishing high-risk MI...