Taxonomic and phenotypic characterization of a novel Providencia species: Providencia lanzhouensis sp. nov.

作者：Yanghui Xiang, Xu Dong, Lan Ma, Dan Cao, Li Yi, Xiuzhi Jiang, Pusheng Xu, Xin Yuan, Kefan Bi, Yiru Zhang, Yuxin Han, Ying Zhang · 发表于：Microbiology Spectrum · 年份：2025 · DOI：10.1128/spectrum.00549-25 · 被引用次数：4 · 研究领域：Bacterial Identification and Susceptibility Testing、Antibiotic Resistance in Bacteria、Genomics and Phylogenetic Studies

ABSTRACT The genus Providencia includes species of ecological and clinical significance, with several members acting as opportunistic pathogens in hospital-acquired infections, particularly urinary tract infections (UTIs). However, overlapping phenotypic traits and genetic similarities among species pose challenges for accurate identification. In this study, we isolated strain PAZ2 from a urine sample of a hospitalized patient in Lanzhou, Gansu Province, China. While initial mass spectrometry analysis identified the strain as Providencia alcalifaciens , comprehensive genomic analysis revealed distinct characteristics suggesting a novel species. Comparative genomic analyses showed average nucleotide identity (ANI) values ranging from 79.92% to 94.73% and digital DNA-DNA hybridization (dDDH) values between 21.2% and 57.6% when compared with known Providencia species. The strain exhibited resistance to multiple antibiotics, including ampicillin, tetracycline, tigecycline, polymyxin B, colistin, trimethoprim-sulfamethoxazole, and ciprofloxacin. Biochemical characterization revealed distinct properties that differentiated PAZ2 from previously described species within the genus. Based on genomic and phenotypic evidence, we propose strain PAZ2 as the type strain of a novel species, Providencia lanzhouensis sp. nov. Geographic distribution analysis indicated P. lanzhouensis presence in five countries, with predominance in China. Notably, comparative genomic analysis revealed that P. ...