Sequence Programmable Order–Disorder Transitions in Supramolecular Assembly of Peptide Nanofibers

作者：Zachary J. Urbach, Naomy Marrufo, Quynh Theresa H., Julie Le, Michał Wierzbicki, Yuanming Song, Khawla Mustafa, Fengbin Wang, Edward H. Egelman, Zhibin Guan, Douglas J. Tobias, Allon I. Hochbaum · 发表于：Journal of the American Chemical Society · 年份：2025 · DOI：10.1021/jacs.5c05344 · 被引用次数：4 · 研究领域：Supramolecular Self-Assembly in Materials、Chemical Synthesis and Analysis、Protein Structure and Dynamics

Protein-protein interactions determine the assembly of complexes that are responsible for numerous key biological processes. The assembly of many natural protein complexes is mediated by post-translational structural changes and environmental stimuli. In this study, we show that incorporation of adjacent lysine residues results in the pH-tunable stability of peptide secondary structure and assembly, allowing for the incorporation of complementary order-inducing motifs. The strategic placement of cysteine pairs in the same peptide sequence results in redox-dependent disulfide staple formation, inducing a transition from random coil to β-sheet conformation and subsequent supramolecular nanofiber assembly from otherwise disordered peptide monomers. Spectroscopic, imaging, molecular dynamics, and kinetic studies highlight the critical role of sequence motif location, oligomerization, and the competitive interplay between intra- and interpeptide disulfide bonding in determining assembly outcomes. We extend this approach to demonstrate phosphorylation-dependent assembly from the design of the same parent peptide sequence, suggesting a general approach to the design of diverse stimulus-responsive peptide sequences for supramolecular assembly. These findings also provide a framework for investigating sequence-dependent pathways in amyloid fiber formation with potential implications for neurodegenerative disease research.