Adipose-derived stem cells alleviate acute pancreatitis by inhibiting ferroptosis and oxidative damage in canines

作者：Yansong Ge, Mingzhen Chen, Meilin Li, Zheng Wang, Ruxin Ding, Zhiying Wan, Enshuang Xu, Jiasan Zheng · 发表于：Stem Cell Research & Therapy · 年份：2025 · DOI：10.1186/s13287-025-04466-4 · 被引用次数：6 · 研究领域：Ferroptosis and cancer prognosis、Pancreatic and Hepatic Oncology Research、Pancreatitis Pathology and Treatment

BACKGROUND: Acute pancreatitis (AP) is a common exocrine pancreatic disease that can lead to systemic inflammatory response syndrome and multiorgan failure in canines. The therapeutic benefits of adipose-derived stem cells (ADSCs) and conditioned medium (CM) and the role in ferroptosis regulation in managing AP in canines (dogs) were investigated in this study. METHODS: /kg) and CM (0.1 mL/kg) were injected intravenously 6 h after surgery, and the roles on ferroptosis and oxidative stress were analyzed. The changing patterns of ferroptosis and oxidative stress were determined in vitro using a lipopolysaccharideinduced cellular inflammation model of AR42J. RESULTS: Ferroptosis occurred in the pancreas during AP, as evidenced by significant iron accumulation, suppressed glutathione peroxidase (GPx)4 expression, and increased transferrin receptor-1 (TFR1), and ferritin heavy chain expression. Treatment with ADSCs and ADSC-CM led to pathological remission and effectively restored abnormal amylase and lipase levels. ADSC-CM showed ferroptosis-alleviating effects similar to that of ADSCs, with reduced iron accumulation and increased GPx4 expression. Furthermore, ADSCs could promote the nuclear translocation of nuclear factor erythroid 2-related factor 2 and initiate the transcription of detoxification enzymes to protect the pancreas from oxidative damage. CONCLUSIONS: ADSCs can protect the pancreas of dogs by inhibiting ferroptosis and oxidative stress via paracrine function, indic...