Cellulase- and pH-Stimulating Selenium Nanopesticide Enhances Antifungal Efficacy to Anthracnose: Action Mechanism and Environmental Safety

作者：Jingyuan Wang, Xiangyang Li, Panpan Chen, Rong Li, Jingwei Hu, Xiangwei Wu, Yi Wang, Hui Li · 发表于：ACS Nano · 年份：2025 · DOI：10.1021/acsnano.5c05437 · 被引用次数：15 · 研究领域：Carbon and Quantum Dots Applications、Polymer-Based Agricultural Enhancements、Nanoparticles: synthesis and applications

Nanopesticides (NPs) provide a sustainable approach to reducing the use of chemical pesticides and mitigating their environmental risks in agriculture. In the present study, a pH and cellulase dual-stimulating system with the loaded pesticide penthiopyrad (PEN) was developed by incorporating hydroxypropyl cellulose (HPC) on the surfaces of mesoporous nanoselenium (MSe). In the measurement of antifungal efficacy of PEN against Colletorichum orbiculare Arx, compared to the neutral environment, i.e., pH = 7 without cellulase, the cumulative release of PEN from HPC@PEN@MSe NPs increased 5.7 and 4.8 times of the amount released at pH 3 and 5, respectively; at pH 7, the presence of cellulase solution substantially enhanced PEN release by 6.1 times more than that without cellulase. The HPC@PEN@MSe NPs demonstrated 3.2-fold of antifungal efficacy greater than the commercial 20% PEN suspension concentrate (20% PEN SC). This dual-stimulating NPs can induce oxidative stress responses and cell structural damage in pathogens, demonstrating a broad spectrum of antifungal activity. Mechanistic investigations illustrated that HPC@PEN@MSe NPs specifically promote the accumulation of reactive oxygen species, including hydroxyl radicals ( • OH), superoxide radicals ( • O 2 – ), and singlet oxygen ( 1 O 2 ) in pathogenic cells by regulating gene expressions relevant to oxidative phosphorylation and lipid metabolism pathways, ultimately leading to eradication of pathogens. In vivo, HPC@PEN@MSe NP...