High Ratio of Manure Substitution Enhanced Soil Organic Carbon Storage via Increasing Particulate Organic Carbon and Nutrient Availability

作者：Xiaoyu Hao, Xingzhu Ma, Lei Sun, Shuangquan Liu, Jinghong Ji, Baoku Zhou, Yue Zhao, Yufeng Zheng, Enjun Kuang, Yitian Liu, Shicheng Zhao · 发表于：Plants · 年份：2025 · DOI：10.3390/plants14132045 · 被引用次数：8 · 研究领域：Soil Carbon and Nitrogen Dynamics、Phosphorus and nutrient management、Agriculture, Soil, Plant Science

Replacing partial chemical fertilizers with organic fertilizer can increase organic carbon input, change soil nutrient stoichiometry and microbial metabolism, and then affect soil organic carbon (SOC) storage. A 6-year field experiment was used to explore the mechanism of SOC storage under different ratios of manure substitution in northeast China, with treatments including chemical fertilizer application alone (nitrogen, phosphorus, and potassium, NPK) and replacing 1/4 (1/4M), 2/4 (2/4M), 3/4 (3/4M), and 4/4 (4/4M) of chemical fertilizer N with manure N. Soil nutrients, enzymatic activity, and SOC fractions were analyzed to evaluate the effect of different manure substitution ratios on SOC storage. A high ratio of manure substitution (>1/4) significantly increased soil total N, total P, total K, and available nutrients (NO3−-N, available P, and available K), and the 4/4M greatly decreased the C/N ratio compared to the NPK. Manure incorporation increased microbial biomass carbon (MBC) by 18.3–53.0%. Treatments with 50%, 75%, and 100% manure substitution (2/4M, 3/4M, and 4/4M) enhanced bacterial necromass carbon (BNC), fungal necromass carbon (FNC), and total microbial necromass carbon (MNC) by 31.9–63.5%, 25.5–107.1%, and 27.4–94.2%, respectively, compared to the NPK treatment. Notably, the increase in FNC was greater than that of BNC as the manure substitution ratio increased. The increasing manure substitution significantly enhanced particulate organic C (POC) and total...