A photosensitive tricarbonyl Mn(I) CO-releasing molecule of 6-mercaptopurine: Genotoxicity and mode of cell death

作者：Ahmed M. Mansour, Rabaa M. Khaled, Krzysztof Radacki, Ola R. Shehab, G. Mostafa, Essam A. Ali, Mona A.M. Abo-Zeid, Mahmoud T. Abo‐Elfadl · 发表于：Journal of Photochemistry and Photobiology A Chemistry · 年份：2025 · DOI：10.1016/j.jphotochem.2025.116587 · 被引用次数：3 · 研究领域：Heme Oxygenase-1 and Carbon Monoxide、Genomics, phytochemicals, and oxidative stress、Porphyrin Metabolism and Disorders

Two photosensitive tricarbonyl manganese(I) complexes [MnX(bpy)(CO) 3 ] 0/+ (X = Br ( 1 ) and 6-mercaptopurine ( 2 ); bpy = 2,2′-bipyridine) were tested for their antiproliferative effects against five normal and malignant cell lines of various origins under dark and 468 nm light exposure. 6-Mercaptopurine is a widely recognised therapeutic drug used to treat human leukaemia. Compound 1 was highly toxic to the THP-1 cell line, with an IC 50 value of 13.6 ± 1.2 μM in light and 3.0 ± 0.1 μM in dark, while 2 had no effect on it. On the other hand, 2 demonstrated slighlty harmful towards HepG2 cells, with IC 50 values of about 100 μM under dark and light conditions. The illumination at 468 nm increased the formation of ROS of both 1 and 2 in comparison to the dark ones. Complex 1 had the greater capacity for apoptosis, which was the predominant mode of cell death. The Comet assay confirmed the genotoxicity of both complexes and the light conditions caused significant genotoxic damage compared to the dark conditions.