Functional diversification of bornyl diphosphate synthase in Lauraceae: An evolutionary framework for borneol-oriented molecular breeding

作者：Qing Ma, Tong Chen, Mei Yang, Wen Xu, Ying Zheng, Haiyan Zhang, Ping Su, Juan Guo, Baolong Jin, Guanghong Cui, Luqi Huang · 发表于：Industrial Crops and Products · 年份：2025 · DOI：10.1016/j.indcrop.2025.121423 · 被引用次数：2 · 研究领域：Plant biochemistry and biosynthesis、Photosynthetic Processes and Mechanisms、Biochemical and biochemical processes

Borneol, a bicyclic monoterpenoid with many pharmacological bioactivities and industrial applications, is biosynthesized through bornyl diphosphate synthase (BPPS) belonging to terpene synthase b (TPS-b) clade. While terpenoids are widely distributed in Lauraceae, high-level borneol production is species-specific, characterizing a unique borneol chemotype with significant economic potential but hindered by low yield (1.2 % dry weight). To elucidate the molecular mechanism by which BPPS drives the formation of borneol chemotypes in Lauraceae species, this study conducted BPPS-directed screening based on public data of 11 transcriptomes and 2 genomes. From 33 TPS-b synthases, 8 BPPS enzymes with distinct activities were identified, expanding the total number of BPPS synthases in Lauraceae to 14. Phylogenetic analysis divided Lauraceae TPS-b family into B-I to B-V clades, where B-III to B-V clades showed correlations with borneol synthesis efficiency, while products from clades I-IV (e.g., 1,8-cineole, linalool, pinene) were closely associated with ecological adaptation of Lauraceae. Collinearity analysis revealed a densely distributed terpene synthase region containing 26 genes on chromosome 7 of the Cinnamomum camphora genome. Notably, BPPS gene clusters (e.g., CcTPS-new ) exhibited rapid evolution through tandem duplication and lineage-specific neofunctionalization in Cinnamomum . These findings elucidated the evolutionary mechanisms and functional diversification of monoterp...