Imperfect hydroxyapatite bioceramics derived from golden pomfret have enhanced osteogenic properties

作者：Changze Zhang, Guangchun Zhao, Xiaorui Wang, Mengting Li, Zhengmao Li, E Yixun, Xiaxin Cao, Maohua Chen, Chaozong Liu · 发表于：Scientific Reports · 年份：2025 · DOI：10.1038/s41598-025-06015-8 · 被引用次数：4 · 研究领域：Bone Tissue Engineering Materials、Facial Trauma and Fracture Management、Collagen: Extraction and Characterization

Abstract Imperfect hydroxyapatite (IHA) bioceramics, which contain defects such as calcium deficiency, carbonate substitution, and metal cation substitution, exhibit improved osteogenic properties. In this study, we used a two-step calcination-hydrothermal process to manufacture two types of golden pomfret bone-derived imperfect hydroxyapatite bioceramics (G-IHA): carbonated calcium-deficient hydroxyapatite (CD-IHA) and carbonated hydroxyapatite (C-IHA). Their composition, surface morphology, zeta potential, degradation capacity, mineralization and osteogenic properties were systematically investigated. The results revealed that G-IHA with a higher defect content, including A-type carbonate substitution and Ca vacancies, had negatively charged surface. As a result, G-IHA surfaces are more favourable to ion exchange and interaction with cations (e.g., Na + , Ca 2+ ) in the microenvironment, which results in improved degradation and mineralization. Specifically, after 28 days of degradation, G-IHA showed significantly higher weight losses (CD-IHA and C-IHA were 17% and 13%, respectively) than commercial hydroxyapatite (CHA; 7%). In addition, G-IHA have a higher better bone-like apatite formation ability, and a higher degree of osteogenic differentiation than CHA. Notably, carbonated calcium-deficient imperfect hydroxyapatite (CD-IHA) exhibited the highest bioactivity and osteogenic capacity as evidenced by its increased alkaline phosphatase activity and improved bone matrix min...