Core–Shell Microneedle Patch with Controlled Sequential Release of CuO2@ZIF-8 Nanoparticles and VEGF for Synergistic Treatment of Infected Wounds

作者：Weihang Peng, Jinze Li, Jiale Cheng, Jinlong Yang, Lingzhi Yang, Haifeng Dong, Xueji Zhang · 发表于：ACS Applied Materials & Interfaces · 年份：2025 · DOI：10.1021/acsami.5c07830 · 被引用次数：9 · 研究领域：Wound Healing and Treatments、Advancements in Transdermal Drug Delivery、Diabetic Foot Ulcer Assessment and Management

Infected wounds are difficult to treat due to biofilm formation, drug-resistant bacteria, and inefficient localized therapy. To address these challenges, we developed a core–shell microneedle (MN) patch that enables sequential delivery of copper peroxide (CuO 2 ) nanoparticles and vascular endothelial growth factor (VEGF) for synergistic healing. CuO 2 was encapsulated in zeolitic imidazolate framework-8 (CuO 2 @ZIF-8) to improve the aqueous stability and regulate reactive oxygen species (ROS) generation. The antibacterial nanocomposite was loaded into a pH-sensitive hyaluronic acid (HA) core layer for rapid release under a weakly acidic wound microenvironment, while VEGF was incorporated into a gelatin methacryloyl (GelMA) shell for sustained delivery. Upon application, the core dissolves to release Cu 2+, Zn 2+, and ROS, disrupting biofilms and killing bacteria. Subsequently, the shell swells and degrades to release VEGF, promoting angiogenesis and tissue regeneration. In a Staphylococcus aureus -infected wound model, the MN patch demonstrated potent antibacterial activity, enhanced neovascularization, and accelerated healing. This programmable, dual-functional MN platform offers an effective strategy for treating infected wounds by integrating infection control with tissue regeneration in a temporally coordinated manner.