A Novel Phospholipase A 1 Gene from Tritipyrum Improves Wheat Early Ripening and Salt Tolerance

作者：Jv Zhang, Shasha Yang, Ming‐Qing Li, Tingting Yuan, Ziqing Yu, Deli Song, Qingqin Zhang, S S Zhang, Guangdong Geng · 发表于：Journal of Agricultural and Food Chemistry · 年份：2025 · DOI：10.1021/acs.jafc.4c12792 · 被引用次数：3 · 研究领域：Plant nutrient uptake and metabolism、Plant Stress Responses and Tolerance、Plant Micronutrient Interactions and Effects

It is important to breed salt-tolerant and early ripening wheat cultivars. Using preliminary transcriptomic data, a phospholipase A 1 gene, TtPLA 1–1, was screened from salt-tolerant Tritipyrum “Y1805”. The TtPLA 1–1 expression level in roots was significantly higher than those in stems and leaves under salt stress. TtPLA 1–1 overexpression (OE) wheat lines showed strong salt tolerance levels and early ripening. The PLA, lysophospholipid, free fatty acid, proline, and soluble sugar contents, as well as root vitality and catalase and peroxidase activities, in OE lines were significantly higher than those in the WT plants under normal and salt-stress conditions. However, the malondialdehyde content decreased. Conversely, TtPLA 1–1 downregulation in “Y1805” using a virus-induced gene silencing system decreased the salt tolerance. According to both transcriptome and yeast two-hybrid analyses, the salt stress-, plant growth-, and flowering-related terms “response to water deprivation”, “response to abscisic acid”, “removal of superoxide radicals”, “carbohydrate metabolic process”, “intracellular signal transduction”, “ l -asparagine biosynthetic process”, “positive regulation of growth”, and “regulation of photoperiodism, flowering” were enriched. An interaction between TtPLA 1–1 and TtRFI2L (RED AND FAR-RED INSENSITIVE 2-like) was revealed. Additionally, TtRFI2L silencing reduced the salt tolerance. In summary, TtPLA 1–1 improved wheat salt tolerance and promoted early maturation...