Phylotaxonomic analysis uncovers underestimated species diversity within the genus Rossellomorea with description of selenium-resistant Rossellomorea yichunensis sp. nov

作者：Jiejie Kong, Zuntao Fu, Qianyang Zhang, Yiqin Liu, Zixuan Luo, Zhigang Zhao, Liangliang Liu, Xiaolong Liu, Dan Xu, Xingjie Li, Dao‐Feng Zhang · 发表于：BMC Microbiology · 年份：2025 · DOI：10.1186/s12866-025-04138-6 · 被引用次数：2 · 研究领域：Mycorrhizal Fungi and Plant Interactions、Plant Pathogens and Fungal Diseases、Plant Diversity and Evolution

A novel Gram-stain-positive, aerobic, motile, rod-shaped strain, designated as YC4-1 T , was isolated from rhizosphere soil of Ageratum conyzoides collected from a selenium-rich area in Yichun, Jiangxi Province, China. Strain YC4-1 T grew optimally at pH 7.0–8.0, 28.0–37.0℃, and 0.5–3.0% (w/v) NaCl; could tolerate up to 5,000 mg/L Na 2 SeO 3 ; and exhibited the ability to biosynthesize selenium nanoparticles. The chemotaxonomic characterization revealed that the polar lipids of strain YC4-1 T were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and an unidentified lipid. The major cellular fatty acids were anteiso-C 15:0 and iso-C 15:0 , and the respiratory quinone was MK-7. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YC4-1 T shared the highest similarity of 99.0% to Rossellomorea arthrocnemi EAR8 T and clusters within the genus Rossellomorea . The genome size was 4,694,377 bp in length, with a DNA G + C content of 41.5%. Phylogenomic reconstruction using the bac120 gene set implied that strain YC4-1 T formed a distinct clade clustering eight unclassified bacteria. The average nucleotide identity (ANI) and the digital DNA–DNA hybridization (dDDH) values between strain YC4-1 T and type strains of the genus Rossellomorea were all below the respective thresholds of ANI and dDDH for species delimitation. Based on phylogenetic analyses and phenotypic and chemotaxonomic characteristics, strain YC4-1 T is concluded to represent a...