Human calculus adhesive-induced periodontitis model recapitulating human periodontitis microbiota in rats

作者：Yuan You, Hongling Wu, Dan-Yan Chen, Siyi Yu, Yue Xu, Ying Tang, Hualing Sun, Cui Huang · 发表于：BMC Oral Health · 年份：2025 · DOI：10.1186/s12903-025-06398-0 · 被引用次数：5 · 研究领域：Oral microbiology and periodontitis research、Endodontics and Root Canal Treatments、Gut microbiota and health

BACKGROUND: Periodontitis is a chronic inflammatory condition characterized by oral dysbiosis. Current animal models of periodontitis using ligation and pathogen inoculation are time-consuming and do not reflect the chronic characteristics of human periodontitis. One of the significant differences is the difference between the current experimental animal periodontitis microbiome and human periodontitis microbiome. This study aims to establish a humanized rat model that mimics clinical conditions and approximates the microbial causes of human periodontitis. METHODS: The human calculus adhesive-induced periodontitis (hCAP) was established by transplanting subgingival dental calculus from periodontitis patients to the subgingival surface of rats using universal adhesive. The study performed stereomicroscopy, micro-CT analysis, hematoxylin and eosin staining, tartrate-resistant acid phosphatase staining, qRT-PCR, ELISA, and 16s rDNA sequencing. RESULTS: The hCAP group showed significant alveolar bone loss and osteoclast formation. The inflammatory factors in gingiva and serum were dramatically increased compared to the placebo rats, revealed by qRT-PCR and ELISA. The oral microbiota of the hCAP group was found to be more similar to that of patients compared with ligation with Porphyromonas gingivalis model, with enhanced colonization of Fusobacterium nucleatum subsp. polymorphum and Prevotella intermedia. The hCAP model also detected pathogenic bacteria of systemic diseases as sh...