Identification and development of KASP markers for genetic loci controlling plant height in bread wheat and evaluation their effects using near isogenic lines

作者：Tao Li, Yanyan Tang, Zhengxi Lin, Baowei Chen, Jinghui Wang, Rui Li, Furong Huang, Juanyu Zhang, Junjun Liang, Haili Zhang, Zehou Liu, Guojun Li, Yang WuYun, Guangbing Deng, Hai Long · 发表于：BMC Plant Biology · 年份：2025 · DOI：10.1186/s12870-025-06820-3 · 被引用次数：2 · 研究领域：Wheat and Barley Genetics and Pathology、Genetic Mapping and Diversity in Plants and Animals、Crop Yield and Soil Fertility

BACKGROUND: Plant height (PH), as a key trait of plant architecture, is the embodiment of biomass accumulation and plays a critical role in determining wheat yield. Excavating diverse PH genes and understanding their pleiotropic effects on important agronomic traits are essential to enrich the PH gene pool and facilitate their further application in breeding. RESULTS: In this study, five stable PH QTLs were identified on chromosome 2B, 4A, 4B, 5A and 6A in a double haploid (DH) population from the cross between two elite wheat cultivars, Chuanmai 42 and Kechengmai 1. The QPh.cib-4B and QPh.cib-6A were major QTL and had a significant additive effect on PH. The QPh.cib-4B was confirmed as the Rht-B1, and QPh.cib-6A might be a new QTL. The major QTLs were further validated in different genetic backgrounds using the Kompetitive Allele-Specific PCR (KASP) markers. Using near-isogenic lines (NILs), QPh.cib-6A was demonstrated to pleiotropically increase plant height (PH), thousand grain weight (TGW), and spike length (SL) without negatively affecting grain number per spike (GNS) or spikelet number per spike (SNS). In contrast, QPh.cib-4B exhibited significant effects on PH but had no influence on TGW, GNS, SL, or SNS. Notably, the frequency of elite haplotype of QPh.cib-6A remains relatively low in Chinese wheat varieties. Thus, the combination of QPh.cib-4B and QPh.cib-6A represents a promising genetic module with considerable potential for high-yield wheat breeding. Expression an...