The mechanism of Guanxin Qiwei dropping pills target Dubosiella to improve atherosclerosis

作者：Yiyi Liao, Jun Li, Yuxin Li, Jing Liu, Tingting Chen, Jingkun Lu, Hongxia Li, Qian Zhang, Yuewu Wang · 发表于：Frontiers in Pharmacology · 年份：2025 · DOI：10.3389/fphar.2025.1633862 · 被引用次数：4 · 研究领域：Gut microbiota and health、Metabolomics and Mass Spectrometry Studies、Traditional Chinese Medicine Analysis

Objective To investigate the therapeutic effects of Guanxin Qiwei dropping pills (GXQW) on atherosclerosis (AS) and to delineate the mechanisms underlying these effects. Methods First, the chemical constituents of GXQW were identified using liquid chromatography-mass spectrometry (LC-MS). In addition, 15 batches of GXQW were used for fingerprint determination. Subsequently, an ApoE −/− mouse model of AS induced by a high-fat diet was established. Lipid deposition, plaque coverage, and collagen fiber content in the aortic arch were evaluated using Oil Red O, H&E, and Masson’s trichrome staining, respectively. Enzyme-linked immunosorbent assay (ELISA) kits were employed to quantify serum oxidative stress markers, inflammatory cytokines, and lipid profiles. Additionally, fecal samples were subjected to 16S rRNA sequencing to investigate the effects of GXQW on intestinal dysbacteriosis. Differential gut microbiota were identified at the phylum-to-genus level. Furthermore, untargeted serum metabolomics was conducted to explore the potential metabolic pathways through which GXQW ameliorated AS. Results A total of 118 chemical constituents were identified in GXQW through database comparison. Compared to the model group, GXQW treatment attenuated lipid deposition and plaque coverage in the aortic arch and mitigated collagen depletion. Fingerprint analysis showed the consistency and stability of the quality of GXQW. Additionally, GXQW reduced total cholesterol (TC) and triglyc...