Disability in mitochondrial aerobic metabolism and Mg2+ transport: linking biomarkers and mechanisms of ischemic heart disease to diesel particulate matter exposure

作者：Ze Zhang, Miao Gan, Juan Ma, Ziyuan Li, Chuer Zheng, Jian Ding, Hao Yin, Xiangcheng Cui, Shan Dai, Rifat Zubair Ahmed, Yong Niu, Shanfa Yu, Xiaoting Jin, Yuxin Zheng · 发表于：BMC Medicine · 年份：2025 · DOI：10.1186/s12916-025-04212-w · 被引用次数：4 · 研究领域：Air Quality and Health Impacts、Climate Change and Health Impacts、Radiation Dose and Imaging

Ischemic heart disease (IHD) is a major cardiovascular health concern. In addition to metabolic and behavioral risks, diesel particulate matter (DPM), with a widely exposed population, is an important external environmental risk factor for IHD. However, the effect biomarkers used to diagnose DPM-caused IHD and underlying mechanisms remain unknown. We investigated the biomarkers and underlying mechanisms of DPM in relation to myocardial hypoxia injury. This study applied a unique population of diesel engine testers with stable DPM exposure. Electrocardiogram examination, echocardiogram examination, serum levels of myocardial enzymes, and 6-min walking test were used for the myocardial risks assessment. A mouse model exposed to occupational environmental DPM dose and in vitro models of DPM-induced myocardial hypoxia injury were used for assessment of mitochondrial aerobic metabolism via the oxygraph-2k system, western blotting, and kits. Ion fluorescence probes, ion supplements, and mitochondrial RNA splicing protein 2 (Mrs2) overexpression transfection were used in further investigations and verifications of the mechanism of mitochondrial Mg2+ deficiency. We identified compromised myocardial mitochondrial aerobic metabolism as a precursor biomarker for the cardiac risk of myocardial hypertrophy and hypoxia injury in DPM exposure. DPM induce mitochondrial Mg2+ deficiency of cardiomyocytes, which in turn disrupt the mitochondrial aerobic metabolism processes, including the trica...