Several groups of LysM-RLKs are involved in symbiotic signal perception and arbuscular mycorrhiza establishment

作者：Yi Ding, Zélie Lesterps, Virginie Gasciolli, Anne-Laure Fuchs, Mégane Gaston, Lauréna Medioni, Annélie de-Regibus, Céline Remblière, Céline Vicédo, Sandra Bensmihen, Jean‐Jacques Bono, Julie V. Cullimore, Guilhem Reyt, Marion Dalmais, Christine Saffray, Solène Mazeau, Abdelhafid Bendahmane, Richard Sibout, Michiel Vandenbussche, Jacques Rouster, Tongming Wang, Guanghua He, Arnaud Masselin, Sylvain Cottaz, Sébastien Fort, Benoît Lefebvre · 发表于：Nature Communications · 年份：2025 · DOI：10.1038/s41467-025-60717-1 · 被引用次数：7 · 研究领域：Mycorrhizal Fungi and Plant Interactions、Legume Nitrogen Fixing Symbiosis、Plant Parasitism and Resistance

Lipo-chitooligosaccharides (LCO) and short-chain chitooligosaccharides (CO) are produced by arbuscular mycorrhizal fungi (AMF) and activate the plant symbiosis signalling pathway, which is essential for mycorrhiza formation. High-affinity LCO receptors belonging to the LysM receptor-like kinase (LysM-RLK) phylogenetic group LYR-IA play a role in AM establishment, but it is unclear which proteins are the plant high-affinity short-chain CO receptors. Here we studied members of the uncharacterized LYR-IB group, and found that they show high affinity for LCO, short- and long-chain CO, and play a complementary role with the LYR-IA receptors for AM establishment. While LYR-IB knock out mutants had a reduced AMF colonization in several species, constitutive/ectopic expression in wheat increased AMF colonization. LYR-IB function is conserved in all tested angiosperms, but in most japonica rice a deletion creates a frameshift in the gene, explaining differences in AM phenotypes between rice and other monocot single LYR-IA mutants. In conclusion, we identified a class of LysM-RLK receptors in angiosperms with unique biochemical properties and a role in both LCO and CO perception for AM establishment. A group of plant receptor-like kinases (LysM-RLKs) with high affinity for short-chain chitooligosaccharides and lipo-chitooligosaccharides (LCOs), play a complementary role in arbuscular mycorrhiza establishment with a sister group of LysM-RLKs specifically recognising LCOs.