Lactiplantibacillus plantarum Lp05 protects against ethanol-induced liver injury in zebrafish through metabolic and microbiota modulation

作者：Yixuan Fan, Yao Dong, Zhonghui Gai, Yanyan Zhang, Mei Han · 发表于：Scientific Reports · 年份：2025 · DOI：10.1038/s41598-025-07111-5 · 被引用次数：6 · 研究领域：Metabolomics and Mass Spectrometry Studies、Alcohol Consumption and Health Effects、Liver Disease Diagnosis and Treatment

This study investigates the protective effects of Lactiplantibacillus plantarum Lp05 on ethanol-induced liver injury in zebrafish (Danio rerio), focusing on its mechanisms related to metabolic pathway regulation and gut microbiota composition. We administered Lp05 to zebrafish exposed to ethanol to assess its impact on liver enzymes and overall liver health. Key metrics included the expression levels of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), activity of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and changes in gut microbiota composition through 16S rRNA sequencing. Metabolomic analyses were conducted to identify affected metabolic pathways. Treatment with Lp05 significantly increased ADH and ALDH expression, enhancing alcohol metabolism and reducing ALT and AST activities, thereby mitigating liver cell damage. Metabolomic analysis revealed significant modulation of biotin and glycerolipid metabolism pathways, crucial for reducing liver injury. Lp05 also altered gut microbiota, increasing beneficial bacteria such as Fluviicola and Delftia, while decreasing pathogenic bacteria like Acinetobacter and Aeromonas. This bacterial modulation contributed to phenylalanine metabolism regulation, which alleviated intestinal inflammation and liver injury. Additionally, Lp05 promoted the degradation of polycyclic aromatic hydrocarbons (PAHs), reducing their health risks. Lp05 exhibits potential therapeutic effects against ethanol-indu...