Mitochondrial genome assembly and comparative analysis of decaploid Camellia hainanica

作者：Shihui Zhang, Yuyan Zhang, Sheng Luo, Jie Gao, Haiyan Hu, Jinping Liu, Wenqiang Wu, Jian Wang, Xiaolong Huang, Hanggui Lai, Dongyi Huang · 发表于：Frontiers in Plant Science · 年份：2025 · DOI：10.3389/fpls.2025.1556379 · 被引用次数：5 · 研究领域：Plant and Fungal Species Descriptions、Genomics and Phylogenetic Studies、Genetic diversity and population structure

Introduction Decaploid Camellia hainanica is a new tea oil Camellia species discovered in recent years that is unique to Hainan. This species has high nutritional and medicinal value and shows strong adaptability in the growth process. Mitochondria play an important role in plant cells and have an independent genetic system. Therefore, assembling and annotating the mitochondrial genome function of decaploid C. hainanica is of great significance. Methods This study successfully assembled the mitochondrial genome of decaploid C. hainanica and comprehensively annotated its functional genes using the Nanopore sequencing platform. Results Results showed that the mitochondrial genome is 902,617 bp in length, with a typical circular structure and a guanine–cytosine content of 45.79%. The genome encodes 64 protein-coding genes and contains a total of 76 genes, including 40 mRNA, 32 tRNA, 3 rRNA, and 1 pseudogene. Tetranucleotide repeats accounted for 38.60% of the simple sequence repeats. Only two genes, atp6 and sdh4 , had a Ka/Ks ratio <1, whereas the Pi value of the sdh3 gene had a maximum of 0.00374 in these regions, suggesting that the sdh3 gene can be used as a molecular marker for the analysis of the mitochondrial genome of C. hainanica . From the relative synonymous codon usage (RSCU) analysis, 29 codons had RSCU values >1, 27 of which (93%) ended in A or U, indicating a bias for A/U endings is present in C. hainanica . During RNA editing, 48.24% (260 loci) of a...