Exosome microsphere/nano silver loaded injectable antibacterial hydrogel augments anti-infection and healing for scald wound

作者：Rongkai Li, Qinbing Qi, Xiaojing Jiang, Zhongfei Gao, Xianrui Xie, Yuqing Zhao, Xiaochen Cheng, Chunhua Wang, Gui‐Ge Hou, Chengbo Li · 发表于：Frontiers in Microbiology · 年份：2025 · DOI：10.3389/fmicb.2025.1550276 · 被引用次数：7 · 研究领域：Wound Healing and Treatments、Burn Injury Management and Outcomes、Electrospun Nanofibers in Biomedical Applications

Infectious scald is recognized as a life-threatening concern in clinical due to easy infection and hard recovery. Exosomes possess cell migration, proliferation and angiogenesis under the containing rich growths factors, which may be a possibility for the treatment of scald chronic wounds. However, the instability and release issues of exosomes limits the storage and therapeutic efficacy. Herein, in this study, exosomes were successfully encapsulated calcium alginate microspheres, effectively avoiding the leakage and inactivation of exosomes. Meanwhile, composite hydrogel (HS-QAF-AgNPs) was fabricated with and quaternized carboxymethyl chitosan (Q-CMC), aldehyde group-modified alginate (ASA) with assisted crosslinking of ferric ion and dopped nano silver (AgNPs). The incorporation of exo-CAMs into the hydrogel protects the exosomes from harsh environmental conditions and extends their diffusion time, thereby guaranteeing a better therapeutic effect. Q-CMC and AgNPs altogether endow the hydrogel system with an inherent antibacterial effect against pathogenic microorganisms. In vitro and in vivo investigations demonstrated that the HS-QAF-AgNPs hydrogel was cytocompatibility, possessed antibacterial and hemostatic properties, promoted cell migration and cell proliferation, stimulated angiogenesis, regulated inflammatory response, delayed apoptosis, enhanced collagen deposition and accelerated wound closure. Thus, the multifunctional HS-QAF-AgNPs hydrogel dressing is expected to...