CcpA-mediated regulation of cellular energy metabolism in the ruminal bacterium Streptococcus bovis

作者：Yunan Weng, Yaqian Jin, Wenze Zhang, Lihong Wang, Hongrong Wang · 发表于：Microbiology Spectrum · 年份：2025 · DOI：10.1128/spectrum.02150-24 · 被引用次数：3 · 研究领域：Bacterial Genetics and Biotechnology、Microbial Metabolic Engineering and Bioproduction、Genomics and Phylogenetic Studies

ABSTRACT This study investigated the cellular energy metabolite profiles of Streptococcus bovis S1. Glucose concentrations of 5 or 50 mM and the presence or absence of ccpA were applied during the cultivation of S. bovis S1. Results revealed the identification of 51 metabolites categorized into seven types: amino acid derivatives, amino acids, coenzymes and vitamins, nucleotides and their metabolites, organic acids and their derivatives, phosphate sugars, and phosphoric acids. Each group exhibited distinct cellular energy metabolite profiles, as evidenced by principal component analysis showing unique metabolite compositions. Orthogonal projections to latent structures discriminant analysis (OPLS-DA) further distinguished between each pair of groups. KEGG prediction indicated that the abundance and enrichment of metabolites were primarily involved in carbon metabolism, amino acid metabolism, and nucleotide metabolomics. Based on the random forest algorithm, glutamine and UDP-GlcNac were identified as biomarkers under varying glucose conditions and in the presence or absence of ccpA . Further analysis reveals that low glucose restricts carbon flux to the Embden-Meyerhof-Parnas (EMP) pathway, while ccpA knockout further reduces flux through this pathway. Glucose and CcpA might regulate fructose-1,6-bisphosphate (FBP) concentration to modulate lactate dehydrogenase and pyruvate formate-lyase enzyme activity, thereby influencing the fermentation direction of pyruvate. In low-gluc...