Maternal Clostridium butyricum supplementation during late gestation and lactation enhances gut bacterial communities, milk quality, and reduces piglet diarrhea

作者：Morakot Nuntapaitoon, Piraya Chatthanathon, Matanee Palasuk, Alisa Wilantho, Jakavat Ruampatana, Sissades Tongsima, Sarn Settachaimongkon, Naraporn Somboonna · 发表于：Computational and Structural Biotechnology Journal · 年份：2025 · DOI：10.1016/j.csbj.2025.06.040 · 被引用次数：6 · 研究领域：Gut microbiota and health、Animal Nutrition and Physiology、Animal Behavior and Welfare Studies

Abstract Experimental objective Diarrhea is a major cause of piglet mortality, often reported associated with maternal gut bacterial communities (microbiota). Maternal supplementation with probiotic Clostridium butyricum during late gestation showed to reduce piglet diarrhea during the suckling period. This study thereby investigated the effects of probiotic supplementation on sow gut (feces) microbiota and their potential microbial metabolisms. Methods Sow and litter performances, including milk compositions and incidences of piglet diarrhea, were recorded from farrowing to weaning of control- supplemented vs. probiotic-supplemented sows. Fecal samples from sows classified as before (Cb=17) and after (Ca=17) probiotic supplementation were analyzed using 16S rRNA gene sequencing and 16S rRNA qPCR, following bioinformatic analyses for alpha-beta diversity, quantitative microbiota, LEfSe (Linear discriminant analysis Effect Size) taxon biomarker analysis, potential microbial metabolism profiles, and statistical correlations with microbial species and clinical data performances. Results Probiotic-supplemented sows demonstrated the greater average piglets born alive and lower mummified fetuses ( P > 0.05), and the statistical higher protein and casein contents in their colostrum ( P < 0.05). Following microbiota analyses, no significant difference was observed in operational taxonomic units (OTUs), Chao1, and Shannon alpha-diversity indices between Cb and Ca samples. Nevertheless...