Biocompatible and Antifouling Linear Poly( N -(2-hydroxypropyl)methacrylamide)-Coated Capillaries via Aqueous RAFT Polymerization Method for Clinical Proteomics Analysis of Non-Small Cell Lung Cancer Tissue by CZE-ESI-MS/MS

作者：Mengqing Yang, Jianhai Yang, Rong Liu, Liping Chen, Danyang Chen, Yu He, Yang Li, Huaying Wang, Keqi Tang, Zhengyan Hu, Zhenbin Zhang · 发表于：Analytical Chemistry · 年份：2025 · DOI：10.1021/acs.analchem.5c02306 · 被引用次数：5 · 研究领域：Microfluidic and Capillary Electrophoresis Applications、Mass Spectrometry Techniques and Applications、Biosensors and Analytical Detection

Capillary coating plays a crucial role in the separation efficiency and reproducibility of capillary zone electrophoresis (CZE). In this study, a linear poly( N -(2-hydroxypropyl)methacrylamide) (LP(HPMA))-coated capillary was prepared by using the surface-confined aqueous reversible addition–fragmentation chain transfer polymerization method. The LP(HPMA)-coated capillary exhibits better biocompatibility and stability compared with the linear poly(acrylamide) (LPA)-coated capillary. Through systematic evaluation, the optimal conditions for fabricating LP(HPMA)-coated capillaries were determined as follows: 1 M HPMA, 2.7 × 10 –3 M 4,4′-azobis(4-cyanovaleric acid), and a reaction time of 4 h. LP(HPMA)-coated capillaries prepared under these conditions exhibited the lowest electro-osmotic flow values of 3.7 × 10 –6 and demonstrated exceptional performance when applied to the analysis of a HeLa cell digest. Following a 20-day treatment with 2 M NH 4 OAc (pH 7), the number of peptides identified using the LP(HPMA)-coated capillary decreased by 18.1% compared to the untreated LP(HPMA)-coated capillary; in contrast, the peptide numbers for the LPA-coated capillary decreased by 26.7% relative to its untreated counterpart. These results indicate that the LP(HPMA)-coated capillary exhibits superior pH stability compared to the LPA-coated capillary within the physiological pH range. The LP(HPMA)-coated capillary was employed in the clinical proteomics analysis of non-small cell lung ca...