The role of senescence-related hub genes correlating with immune infiltration in type A aortic dissection: Novel insights based on bioinformatic analysis

作者：Wei He, Panli Tang · 发表于：PLoS ONE · 年份：2025 · DOI：10.1371/journal.pone.0326939 · 被引用次数：2 · 研究领域：Connective tissue disorders research、Aortic Disease and Treatment Approaches、Congenital heart defects research

BACKGROUND: Stanford type A aortic dissection (AAD) is a fatal disease that confers extremely high morbidity and mortality. Cellular senescence, characterized by a permanent cell cycle arrest, has been implicated in the onset and progression of cardiovascular disease and immune cell infiltration has been observed in the aortic walls of dissected specimens. However, the precise mechanisms through which senescent cells interact with immune infiltration to contribute to the development and progression of AAD remain unclear. METHODS: Cellular senescence-related genes (SRGs) were identified via the CellAge database. Patient and normal control datasets (GSE52093 and GSE190635) were retrieved from the Gene Expression Omnibus (GEO) database, with GSE190635 serving as the validation set. Differentially expressed genes (DEGs) linked to AAD were determined from the GSE52093 dataset. We intersected SRGs with DEGs to identify differentially expressed senescence-related genes (DESRGs), which were subsequently analyzed for Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and protein-protein interactions (PPI). Hub DESRGs were selected based on their connectivity degree and diagnostic genes were further refined via gene expression level evaluation and receiver operating characteristic (ROC) curve analysis. Additionally, a miRNA-gene network involving hub DESRGs was constructed. Finally, CIBERSORT was employed to analyze the compositional patterns of the...