Mesoporous silica nanoparticles enhance the toxicity of chlorantraniliprole to Spodoptera frugiperda by possibly inhibiting energy metabolism and chitin protein synthesis

作者：Suman Zong, Dejin Xu, Yiping Jiang, Xiaofeng Wang, Keyan Zhu‐Salzman, Xin Zhang, Jing Zhao, Liubin Xiao, Leigang Zhang, Guangchun Xu, Aiguo Gu, Hao Hong, Linquan Ge, Yongan Tan · 发表于：Insect Science · 年份：2025 · DOI：10.1111/1744-7917.70106 · 被引用次数：7 · 研究领域：Polymer-Based Agricultural Enhancements、Legume Nitrogen Fixing Symbiosis、Insect Resistance and Genetics

Nanosized formulations are an important means to develop effective pesticide molecules against target pests with improved environmental safety. In this study, we constructed a nanodelivery system using mesoporous silica nanoparticles (MSNs) carrying chlorantraniliprole (CLAP). The drug loading rate was determined using liquid chromatography, and CLAP@MSNs were characterized and analyzed. Adhesion was measured by contact angle and surface tension, and UV resistance was assessed. The transport of CLAP@MSNs within plants was observed using confocal fluorescence microscopy. Additionally, larval bioassay experiments and RNA-seq analyses were conducted on CLAP@MSNs. The results indicate that CLAP (35.6% w/w) has been successfully loaded onto MSNs. CLAP@MSNs appeared to have structure and size similar to MSNs. CLAP@MSNs showed effective adhesion to the surface of corn leaves and stems and also resisted to UV photolysis. Bidirectional delivery of fluorescently labeled CLAP@MSNs through vascular bundles in corn. When administered to Spodoptera frugiperda, mortality in CLAP@MSNs-treated larvae increased whereas weight and developmental period decreased significantly compared with larvae treated with CLAP alone. RNA-seq analysis revealed that oral administration of CLAP@MSNs led to the downregulation of genes associated with drug metabolism, energy metabolism, and chitin protein synthesis, thereby inhibiting the growth and development of insects. Interestingly, CLAP@MSNs exerted no harm...