Genomic analysis and antimicrobial resistance in human- and poultry-derived Campylobacter jejuni isolates from Hangzhou, China

作者：Mingfang Yang, Xin Wang, Zheng LuPing, Yongli Zhu · 发表于：Frontiers in Microbiology · 年份：2025 · DOI：10.3389/fmicb.2025.1599555 · 被引用次数：3 · 研究领域：Salmonella and Campylobacter epidemiology、Viral gastroenteritis research and epidemiology、Bacteriophages and microbial interactions

Campylobacter jejuni ( C. jejuni ) is a zoonotic pathogen and is the most prevalent foodborne pathogen globally. The increasing antimicrobial resistance and gene mutation pose a threat to public health and trigger us to enhance surveillance. This study illustrated the antimicrobial resistance profiles, virulence factors, and multilocus sequence typing (MLST) profiles of 47 C. jejuni isolates collected from human stool and raw poultry meat samples between 2022 and 2023 in Hangzhou, China. Notably, 88.9% (16/18) human-derived and 82.9% (24/29) poultry-derived C. jejuni displayed multidrug resistance (MDR) profiles, nalidixic acid + ciprofloxacin + tetracycline was prevalent among them. Relatively high rates of resistance to florfenicol were observed in C. jejuni , 38.9% (7/18) from human sources and 44.8% (13/29) from poultry sources. Comprehensive Antibiotic Resistance Database (CARD) and ResFinder database showed gyrA ( T86I ) was the dominant factor in quinolones resistance while a rare gyrA ( T86V ) was found in one poultry-derived C. jejuni . All 37 tetracycline-resistant C. jejuni strains harbored the tet(O) gene. All 20 florfenicol-resistant C. jejuni did not have any related genes. Several key virulence factor genes associated with adherence ( cadF, pebA, jlpA , and porA ), invasion ( CiaB and CiaC ), capsule biosynthesis/transport genes ( kpsF, kpsD, kpsE, kpsM, kpsC , and Cj1419c ) and lipooligosaccharide (LOS) biosynthesis genes ( Cj1135, waaV, waaF, htrB, gmhA , and...