Genome-Wide Association Study for Weight-Related Traits in Scylla paramamosain Using Whole-Genome Resequencing

作者：Lin Chen, Yaodong Zhang, Puping Jia, Siyi Zhou, Qionghui Qin, Weiren Zhang, Kewei Huang, Xiaopeng Wang, Haihui Ye · 发表于：Animals · 年份：2025 · DOI：10.3390/ani15131829 · 被引用次数：5 · 研究领域：Crustacean biology and ecology、Invertebrate Immune Response Mechanisms、Aquaculture Nutrition and Growth

Weight traits serve as key economic indicators for assessing growth performance and commercial quality in the mud crab Scylla paramamosain, yet the genetic basis of these traits remains poorly characterized. Here, we performed whole-genome resequencing on 323 individuals and conducted genome-wide association studies (GWAS) on five weight-related traits: (1) body-related traits, including body weight (BW), trunk weight (TruW), and weight excluding chelae (WEC); (2) appendage-related traits, containing appendage weight (AppW) and cheliped weight (CheW). Significantly associated SNPs were primarily enriched on chromosomes 15, 22, 25, and 36. For body-related traits, we identified 45 shared candidate SNPs and 175 common candidate genes; appendage-related traits revealed 71 shared candidate SNPs, and 229 common genes were identified; and across all five traits, there were 9 shared candidate SNPs and 49 common genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that shared functional terms/pathways among the five traits were mainly related to metabolism, development, and immunity. Body-related traits exhibited more unique GO terms and KEGG pathways associated with metabolism and immunity, whereas appendage-related traits showed some unique GO terms and KEGG pathways involved in development and morphogenesis. Among the candidate genes, we identified multiple genes associated with growth and development, metabolism, and immune responses. Fo...