Comprehensive Analysis of Faba Bean AP2/ERF Genes Suggests Potential Roles of VfAP2‐1 and VfERF‐99 in Abiotic and Biotic Stress Responses

作者：Lemeng Dong, Ting Huang, Shuo Han, Xiaowen Han, Junliang Yin, Lu Hou, Yujiao Liu · 发表于：Physiologia Plantarum · 年份：2025 · DOI：10.1111/ppl.70356 · 被引用次数：5 · 研究领域：Genetic and Environmental Crop Studies、Plant-Microbe Interactions and Immunity、Plant Reproductive Biology

ABSTRACT The AP2/ERFs not only participate in regulating signal networks, but they also play important roles in the process of plant growth and stress response. However, systematic research of AP2/ERF in Vicia faba is lacking. In this study, VfAP2/ERF was systematically identified and their characteristics were comprehensively analyzed. In total, 145 VfAP2/ERFs were identified, which were unevenly distributed across six chromosomes, and according to phylogenetic relationships, VfAP2/ERFs could be classified into five subgroups. Cis ‐elements analysis showed that VfAP2/ERF promoters harbored numerous elements functionally relating to light response, plant hormone, abiotic stress response, and plant growth and development response. Expression profiling analysis indicated that VfAP2/ERFs were broadly expressed during growth and development, and were responsive to drought and salt stresses. RT‐qPCR revealed that six VfAP2/ERF genes were upregulated under drought and salt stress. Inoculation assay showed that VfAP2‐1 and VfERF‐99 could enhance resistance to pathogens. Further research shows that VfAP2‐1 and VfERF‐99 positively influence ROS homeostasis, resulting in the accumulation of H 2 O 2 and O 2 − under abiotic and biotic stresses, which inhibited the colonization of pathogens. Additionally, VfAP2‐1 and VfERF‐99 could significantly increase the content of chlorophyll a , carotenoids, and total chlorophyll, suggesting their possible roles in promoting photosynthesis. This stu...