Ecological effects of water and fertilizer addition on poplar-planting soil

作者：Yimin You, Shitong Li, Li Xiao, Liran Wang, Hongxing Wang, Luping Jiang, Yanhui Peng, Zhongyi Pang, Xiyang Zhao · 发表于：mSystems · 年份：2025 · DOI：10.1128/msystems.00501-25 · 被引用次数：4 · 研究领域：Microbial Community Ecology and Physiology、Soil Carbon and Nitrogen Dynamics、Genomics and Phylogenetic Studies

ABSTRACT While irrigation and fertilization are basic cultivation practices in poplar plantations on a global scale, the impact of these practices on the environment is not well understood. Here, we demonstrate that water-urea addition and water-compound fertilizer addition differentially impact soil ecosystems. We report that water-fertilizer addition did not significantly alter taxonomic diversity indices, but it did drive significant shifts in microbial community composition, reflected by changes in the relative abundance of specific taxa and their functional profiles. Water-urea addition reduced Proteobacteria and Actinobacteria in non-rhizosphere soils while increasing Acidobacteria and Chloroflexi. In contrast, water-compound fertilizer additions increased Proteobacteria and Actinobacteria dominance in rhizosphere soils. Water-fertilizer addition changed microbial composition and functional gene abundance linked to nitrogen and sulfur cycling. Water-urea treatment enriched denitrification genes and dissimilatory nitrate reduction genes ( napABC ) in rhizosphere soil, while water-compound fertilizer treatment enhanced nitrification ( amoABC , HAO ) and denitrification gene abundance in both soils. For sulfur (S) cycling, water-urea treatment favored thiosulfate oxidation genes, whereas water-compound fertilizer treatment increased assimilatory sulfate reduction genes. Multi-omics integration linked these microbial dynamics to metabolic reshaping—water-urea increased lipi...