Prevalence, antimicrobial resistance and biofilm forming ability of Escherichia coli in milk, animal handlers and slurry samples from dairy herds of Punjab, India

作者：Deepthi Vijay, Jasbir Singh Bedi, Pankaj Dhaka, Randhir Singh, Jaswinder Singh, Anil Arora, Jatinder Paul Singh Gill · 发表于：Frontiers in Veterinary Science · 年份：2025 · DOI：10.3389/fvets.2025.1553468 · 被引用次数：10 · 研究领域：Bacteriophages and microbial interactions、Antibiotic Resistance in Bacteria、Microbial infections and disease research

Antimicrobial-resistant Escherichia coli serves as an indicator for monitoring the occurrence of antimicrobial resistance (AMR) at the human, animal, and environmental interface. The present study employs a ‘One Health’ framework to determine the prevalence, phenotypic and genotypic characterisation of the AMR profile and biofilm forming ability of E. coli isolates from milk, hand swabs and slurry samples of 405 dairy herds in Punjab, India. An overall prevalence of E. coli was 34.3% (139/405) (95% CI: 0.30 to 0.39) from pooled milk samples, 9.1% (37/405) (95% CI: 0.06 to 0.12) from the hand swabs of animal handlers and 64.4% (261/405) (95% CI: 0.60 to 0.69) in the slurry samples. Multidrug resistance (resistance to 3 or more classes of antimicrobials) was exhibited by 24.4% (34/139) of E. coli isolates from milk, 40.5% (15/37) from hand swabs, 60.5% (158/261) from slurry samples. Moreover, of the E. coli isolates, 11.51% (16/139) from milk, 24.32% (09/37) from hand swabs and 31.42% (82/261) from slurry samples were resistant to 5 or more antimicrobial classes. On molecular characterisation, 19.4% (27/139) of E. coli isolates from milk, 37.8% (14/37) from hand swabs, and 33.3% (87/261) from slurry samples harboured various genes. Principal component analysis and Shannon-Wiener diversity indices highlighted varying β -lactamase (ESBL/AmpC β-lactamase) gene distributions across samples, with milk exhibiting the highest diversity. Logistic regression analysis revealed a signific...