Metabolomic response of Zizania latifolia to low-temperature stress and identification of the bZIP transcription factor family

作者：Yushi Jiang, Xing Chen, Fang Wang, Xinze Li, Zhenyuan Qin, Shuang Fan, Ning Yan, Yanning Xie, Rengui Zhao · 发表于：GM crops & food · 年份：2025 · DOI：10.1080/21645698.2025.2510715 · 被引用次数：4 · 研究领域：Plant Gene Expression Analysis、Plant Molecular Biology Research、Plant Stress Responses and Tolerance

Cold stress severely impacts crop production, making it crucial to dissect the metabolic and transcriptional regulatory mechanisms of cold-resistant plants for breeding cold-tolerant varieties. This study systematically explored the response mechanism of Zizania latifolia to cold stress by integrating widely targeted metabolomics and genome-wide analysis for the first time. Metabolomics analysis revealed that 690 out of 810 metabolites showed significant differences after cold treatment at 4°C, with significant enrichment of flavonoids, amino acid derivatives, and alkaloids, involving key pathways such as antioxidant defense, osmotic adjustment, and signal transduction. This indicates that Z. latifolia copes with cold stress through the coordination of secondary and primary metabolism. A total of 115 bZIP transcription factors (ZlbZIPs) were identified from the Z. latifolia genome, with 18 genes located in known cold-resistant quantitative trait locus (QTL) intervals. Four cold-tolerant candidate genes were screened through collinearity analysis with the rice genome. Expression analysis showed that ZlbZIP005, ZlbZIP075, and ZlbZIP084 were significantly upregulated (29.17–4.10 fold) at 24 hours of cold treatment, and their promoter regions with high-density G-box elements implied strong cold response potential. Phylogenetic and evolutionary analyses showed that the bZIP family of Z. latifolia is highly homologous to that of rice but exhibits subfamily-specific expansion (such ...