Spatial ratio of two fungal genotypes content of Naematelia aurantialba and Stereum hirsutum in nutritional growth substrate and fruiting bodies reveals their potential parasitic life cycle characteristics

作者：Jin-Xin Lan, Yongkang Zhang, Yingli Cai, Xiaofei Shi, K. Zhang, Jiacheng Huang, Chengmo Yang, Xinhua He, Fuqiang Yu, Wei Liu · 发表于：Journal of Agriculture and Food Research · 年份：2025 · DOI：10.1016/j.jafr.2025.102101 · 被引用次数：3 · 研究领域：Plant and fungal interactions、Fungal Biology and Applications、Mycorrhizal Fungi and Plant Interactions

The JinEr (Golden Era) mushroom is a rare and nutritionally valuable macrofungus, prized for its unique bioactive compounds and culinary properties. It is believed to form heterogeneous fruiting bodies comprising two fungal species: Naematelia aurantialba and Stereum hirsutum . While, research on their interaction, particularly regarding the cellular composition ratio within the substrate and fruiting body was limited. In this study, through whole-genome comparisons of 56 species, we identified candidate genes with intra- and inter-specific variation. Nine species-specific fragments were selected, with four primer pairs validated after evaluating amplification efficiency and melting curves. By comparing Ct values from four species-specific primer pairs, we quantified the relative proportions of both genotypes and revealed their striking spatial partitioning. The outer apex of the fruiting body showed the closest proportions ( S. hirsutum : N. aurantialba = 1.51:1), with S. hirsutum dominance increasing progressively downward to 3.97:1 (middle of fruiting body) and 6.52:1 (base of fruiting body). Across all fruiting body sections, S. hirsutum genotypes predominated (77.12% of total content), demonstrating that most edible tissue derives from this species rather than the prized N. aurantialba . Below the fruiting body, genotype ratios exhibited exponential divergence: increasing to 16.28:1 at 3 cm below the base, and reaching 520.92:1 at the substrate midpoint, with N. aurantia...