Triple-Cue-Guided Multichannel Hydrogel Conduit to Synergistically Enhance Peripheral Nerve Repair

作者：Yuting Cai, Peng-Hui Wang, Yaxuan Li, Tsz Wing Tang, Linjie Zhang, Hongxin Shu, Hoilun Wong, Yuyin Li, Jingwei Li, Ana Claudia Arias, Chenguang Zhang, Guorui Jin, Qun Huang, Zhengtang Luo · 发表于：ACS Nano · 年份：2025 · DOI：10.1021/acsnano.5c03215 · 被引用次数：21 · 研究领域：Nerve injury and regeneration、Nerve Injury and Rehabilitation、Hydrogels: synthesis, properties, applications

Multichannel nerve guidance conduits (NGCs) have demonstrated superior efficacy in the regeneration of large nerve defects. Here, we present the incorporation of three synergistic guiding cues into a single multichannel hydrogel conduit: topographical guidance, a conductivity gradient, and a nerve growth factor (NGF) gradient. The aligned hydrogel conduit is fabricated through directional lyophilization of a graphene oxide (GO)/poly(vinyl alcohol) (PVA) solution. The conductivity gradient is achieved via the dopamine-induced reduction of GO, during which amino groups with a concentration gradient are simultaneously generated, facilitating the eventual formation of an NGF gradient. In vitro experiments validate the excellent guidance effect and promotion of neuritogenesis by the NGF-gradient/aligned PVA/reduced graphene oxide (rGO)/polydopamine (PDA)/heparin hydrogel conduit (denoted as NGF-AGHC) on PC12 neuronal cells. Furthermore, in vivo testing reveals that NGF-AGHC exhibits a stronger longitudinal attraction to axons and promotes remyelination. Additionally, functional recovery assessments, histological analyses, and morphological evaluations all indicate that NGF-AGHC significantly enhances peripheral nerve regeneration, with performance comparable to that of the autograft group. Altogether, through a straightforward preparation method integrating topographical guidance, conductivity gradients, and NGF gradients, our NGCs offer a promising approach to peripheral nerve re...