Regulatory TCRαβ+ Double Negative T Cells Suppress γδ T Cells and Alleviate Colitis

作者：Mingyang Li, Lehan Pan, Yuxi Zhang, Shiyang Huang, Yue Tian, Dan Tian, Dong Zhang · 发表于：Cellular and Molecular Gastroenterology and Hepatology · 年份：2025 · DOI：10.1016/j.jcmgh.2025.101553 · 被引用次数：1 · 研究领域：Immune Cell Function and Interaction、T-cell and B-cell Immunology、IL-33, ST2, and ILC Pathways

BACKGROUND AND AIMS: double-negative T (DNT) cells play pivotal roles in orchestrating the homeostasis of various organs; however, their specific influence on colitis has yet to be thoroughly elucidated. METHODS: Single-cell RNA sequencing and adoptive transfer were used to delineate the distinct signatures of DNT cells and to investigate their role in the context of colitis, respectively. RESULTS: γδ T cells, thereby collaboratively enhancing the cytotoxicity of DNT cells against γδ T-cell populations in concert with NKG2D, ultimately promoting the remission of colitis. Furthermore, mice that received allogeneic DNT cells exhibited ameliorated colitis without inducing graft-versus-host disease. CONCLUSIONS: γδ T-cell responses. The adoptive transfer of DNT cells could be a novel therapeutic strategy for inflammatory bowel disease.