Nervous system-wide single-cell morphology atlas of excitatory and inhibitory neurons in larval zebrafish

作者：Xu-Fei Du, Zhifeng Yue, Ming-Quan Chen, W Li, Tian-Lun Chen, Han-Yang Hu, Hong-Li Wan, Ting-Ting Zhao, Yongwei Zhong, Xinyu Ning, Xiu-Dan Zheng, Hong-An Ren, Ruiqi Wang, Rong Zhao, Xiao-Lan Peng, Zhiming Jia, Li-Jun Chen, Chenxi Jin, Jia-Wen Huang, Wei Deng, Liu-Qin Qian, Li Wang, Mei-Yu Zheng, Wei Zhang, Xiulian Shen, Xiu-Lian Shen, Xiao-Ying Qiu, Qi-Meng Zhao, Dan-Yang Li, Si-Jia Wang, Yuchen Gong, Yan Li, Mu Huo Yu, Peng Ji, Jie He, Yufan Wang, Jiulin Du · 发表于：bioRxiv (Cold Spring Harbor Laboratory) · 年份：2025 · DOI：10.1101/2025.06.06.658008 · 被引用次数：8 · 研究领域：Zebrafish Biomedical Research Applications、Neuroscience and Neural Engineering、Neural dynamics and brain function

SUMMARY Single-neuron morphology mapping is fundamental for deciphering brain architecture and function, yet in vertebrates it remains limited in spatial coverage and cell-type information. Here we present a nervous system-wide single-neuron morphology atlas of larval zebrafish with annotated excitatory/inhibitory identity and dendrite-axon polarity. The atlas comprises >20,000 reconstructed neurons from >13,400 animals, spanning the central and peripheral nervous system and representing ∼24.5% of the total excitatory and inhibitory neurons in the brain, all integrated into a common physical space with neuroanatomical parcellations. We define >500 excitatory and inhibitory neuronal morphotypes and construct putative inter-cell-type and interregional connectomes. Projection and morphotype analyses reveal distinct laterality and directionality biases and modular innervation patterns in excitatory and inhibitory neurons, while network analyses uncover fundamental connectivity motifs, hubs, and sensorimotor pathways. Accessible through custom-built interactive platform ZExplorer, this atlas provides a foundational resource for multimodal data integration, circuit analysis, computational modeling, and hypothesis-driven research.